Uncultured adipose tissue-derived stromal cells as an MSC alternative for hepatitis

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Researchers at Japan’s Kanazawa University Hospital have investigated the use of uncultured adipose tissue-derived stromal cells (u-ADSCs)uncultured adipose tissue-derived stromal cells (u-ADSCs) for treatment of hepatitis in an animal model. The cells were found to treat the hepatitis effectively, in part by inhibiting the expression of chemokines and cytokines.

The team decided to investigate the therapeutic use of u-ADSCs due to the volume of mesenchymal stem cells that they harbor. Freshly harvested, autologous u-ADSCs are therefore a potential alternative to isolated mesenchymal stem cells. When examining the characteristics of u-ADSCs collected from mice, 10% to 20% were found to express the CD45 leukocyte-related antigen. CD68, which is a marker of macrophages, was expressed by 50% of CD45+ u-ADSCs, and approximately 90% of CD68+ CD45+ cells expressed the CD206 antigen, a marker of inhibitory M2-type macrophages.

As well as inhibiting inflammatory cytokines, the u-ADSCs were also found to inhibit the proliferation of splenocytes. This aided the repair of inflamed liver tissue in the murine subjects. The team concluded that u-ADSCs, and particularly the CD45+ subset act genetically and phenotypically as m2-type macrophages.

Source:The CD45+ fraction in murine adipose tissue-derived stromal cells harbor immune-inhibitory inflammatory cells. Nasti, A. et al., Eur J Immunol. Sept 2017DOI