Allogeneic MSCs increase expression of angiogenic factors in ischemic mice

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Researchers at Vietnam’s University of Science have completed an investigation into the role of allogenic adipose-derived mesenchymal stem cells (ADMSCs) for the treatment of damaged vascular muscles or tissues. The preclinical study in mouse models concluded that grafted ADMSCs have a significantly positive effect on stimulating the expression of genes related to new blood vessel formation.

Hindlimb ischemic mice were used as the in vivo model for the study, two groups of these had cuts administered to the proximal end of the femoral blood vessel with one group being treated with ADMSCs and the other control group with phosphate buffer saline (PBS). A UV-Vis system was used to analyse the migration of ADMSCs, this was possible due to the prior labelling of the cells with green fluorescent protein (GFP).

The team evaluated the expression of angiogenic genes, along with cell migration and length of post-graft existence. The results showed that compared with the control group, the expression of several genes involved in vessel formation increased – these included Flt-1, Flk-1, and Ang-2. Cell migration was tracked to other damaged areas in the hindlimb and persisted for 7 days.

Published in the Advances in experimental medicine and biology journal, the results from this study corroborate the interest in this emerging field, and point towards further investigation into the grafting of ADMSCs for vascular renewal.

Source: Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Enhances the Expression of Angiogenic Factors in a Mouse Acute Hindlimb Ischemic Model. Vu NB et al., Advances in experimental medicine and biology July 2017. DOI