Scalable ultrafiltration/diafiltration process of clarified pDNA using T-series cassettes with Omega™ membrane

Nucleic Acid Insights 2024; 1(1), 29

DOI: 10.18609/nai/2024.005

Published: 23 February
Angel Lorenzo, Adam Armengol


In the version of this FastFacts poster initially published, Adam Armengol was listed as the sole author. However, the poster was in fact co-authored by Angel Lorenzo. This error has been corrected in the HTML and PDF versions of this article as of March 6, 2024.

Watch the video or view the poster to learn:

  • Why plasmid compaction promoted by use of high-salt buffers is a key challenge for the UF-DF step of the pDNA production process

  • How plasmid compaction can result in pDNA passage through the UF membrane, resulting in low yields

  • Why Cytiva’s Centramate™ and Centrasette™ cassettes with Omega polyethersulfone (PES) membrane are an effective technology for the concentration and diafiltration of pDNA when buffer characteristics promote plasmid compaction.