Scalable chromatographic strategies for mRNA and saRNA purification from IVT

The potential of RNA-based therapies continues to be explored in the vaccine segment, which has seen strong development during the COVID-19 pandemic, and beyond. Besides messenger RNA (mRNA), emerging RNA modalities such as self-amplifying RNA (saRNA) and circular RNA are gaining interest for their ability to reduce dosage or expand into new therapeutic areas. However, the structure and properties of these new RNA platforms bring challenges in production and purification.

This webinar focuses on affinity purification of RNA, and expands into development of new chromatography tools for purification of mRNA and saRNA. Case studies will delve into purification of mRNA, highlighting some of the challenges faced along the way. The webinar will cover optimizing affinity chromatography binding capacity up to 6 mg/mL, using non-affinity chromatographic methods for mRNA and saRNA (10,000 nt), and touch on supporting topics in the mRNA production workflow, such process monitoring, and reduction of dsRNA.

Join this webinar to learn about:

• Ins-and-outs of affinity chromatography for mRNA purification
• Challenges faced with purification of self-amplifying RNA
• Alternatives to affinity chromatography and their use in purification of mRNA and saRNA
• How to implement efficient analytics to support process development and in-process monitoring in mRNA purification

Tomas Kostelec

Product Manager, pDNA, mRNA at Sartorius BIA Separations

Tomas Kostelec holds a Master’s degree in Chemistry from University College London. He has been with BIA Separations, now part of Sartorius for 8 years. His work includes analytics and purification of viruses and nucleic acids in support of process development activities, and for new product development. Before his current role he was heading the technical support team and supported customers in implementation of monoliths during tech transfer activities. Tomas is now Product Manager for nucleic acid chromatography at Sartorius BIA Separations.

Watch now

SPEAKERS

Tomas Kostelec

Product Manager, pDNA, mRNA at Sartorius BIA Separations

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