Optimizing LNP formulations: leveraging monolithic columns for precise insight and improvement strategies

Thursday 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

Sponsor

Optimizing LNP formulations: leveraging monolithic columns for precise insight and improvement strategies

Lipid nanoparticles (LNPs) have emerged as the foremost non-viral carriers for therapeutics and vaccines, capable of encapsulating various payloads. LNP carriers provide a significant advantage over traditional viral delivery systems, enabling modularity, speed, and better scalability. However, they pose significant challenges in terms of their purification and characterization.

Post-formulation, LNPs require downstream processing to make the formulation applicable for in vivo applications and monitoring of CQAs. Analytical methods for process monitoring and QC analytics of LNPs are mostly offline and require disassembling the particles into individual components beforehand. The process of formulation and purification can be monitored by chromatographic methods using monolithic columns. More specifically, two-dimensional chromatographic analytics enables direct analysis of LNP formulation, without any sample pre-treatment. Multiple detectors allow for the determination of encapsulation efficiency, nucleic acid content, nucleic acid integrity, and separation of co-encapsulated cargos in a single chromatographic run. Additional chromatographic methods that further address the heterogeneity of LNPs by utilizing different monolithic column chemistries and buffer effects are presented.

Attend this webinar to:

Learn how to capitalize on the advantages of scalable methods for separation of different biomacromolecules
Understand the benefits of highly tunable chromatographic methods
Discover how to enable a high degree of automation in LNP downstream processing
Optimize LNP formulations and enable their comprehensive analysis

Nejc Pavlin

Project Manager at Sartorius BIA Separations

Nejc has been a Project Manager in the analytics development department at Sartorius BIA Separations for 3 years. Nejc has extensive experience in analytics development for different biological molecules such as pDNA, mRNA, LNPs, etc., intended for therapeutic use. He managed several projects for clients, mainly in the field of new generation gene therapy products. He holds a PhD in Chemistry from the University of Ljubljana, Slovenia.

Tristan Kovačič

Senior Research Scientist at Sartorius BIA Separations

Tristan is a Scientist in the process analytics development department of Sartorius BIA Separations. He holds an MSci in Chemistry from Imperial College London. He has extensive experience in the non-viral delivery field, especially analytics, formulation, and downstream development of LNPs.