Elevate LNP optimization with Sunshine and Stunner

Live30 webinars are thirty-minute presentations designed to update you on the latest innovations, applications, and data in a fast yet interactive format.

Problem

When working with LNPs, dialing in your process and flow parameters can be a tough nut to crack with one-by-one methods, and when you’re finally ready for clinical production, you’re stuck with all the re-optimization that comes with switching equipment. Tack on all the sample hungry experiments needed to I.D. your final lipid formulation, ideal flow rate, and buffers to improve stability, and you’re looking at tons of wasted time and resources.

Solution

Sunshine is the LNP production tool that beams through all the challenges of process development, LNP optimization, and continuous production. Sunshine’s flexibility across mixer types and process conditions is ideal for optimization work – while producing enough LNPs for any assay. Stunner handles all the analysis on particle size, PDI, particle count, and complete quantification of total RNA in less than 2 minutes from just 2 µL per sample. Stunner’s LNP applications detect aggregates like no other instrument and eliminates half the work of encapsulation efficiency assays.

Proof

In this seminar, we’ll show you how Sunshine and Stunner make the perfect pair when it comes to accelerating LNP optimization and development. We’ll compare multiple LNP formulations, flow rates, and cryoprotectant buffer conditions to see how size, polydispersity, aggregation, and RNA concentration stack up after production and after freeze-thaw cycling. Come see how Stunner’s unique approach to LNP sizing can help you spot aggregates you couldn’t see before and can help you save time, material, and hassle in your LNP workflow.

• How to identify the best LNP candidates and conditions for optimization and further development
• How lipid formulation, total flow rate, and buffer condition play a role in LNP size and freeze-thaw stability
• How to develop LNP processes that are consistent across different LNP production volumes
• How to evaluate LNP conditions in high-throughput for multiple parameters simultaneously

Kevin Lance

Director, Marketing at Unchained Labs

Kevin Lance covers the Stunner portfolio globally at Unchained Labs. His expertise includes biophysical characterization of proteins, nucleic acids, viral vectors, and LNPs. Prior to Unchained Labs, his research experience covered the fields of sustained antibody delivery and nanotechnology. He earned his Ph.D. in Bioengineering from the joint UC Berkeley – UCSF Bioengineering Graduate Program.