mRNA characterization - from 5’ cap to poly (A): what IPRP-LC–MS can tell you

Live30 webinars are thirty-minute presentations designed to update you on the latest innovations, applications, and data in a fast yet interactive format.

mRNA is a biotherapeutic modality primarily used in vaccines, and undergoes a complex manufacturing process involving in vitro transcription. This process yields mRNA molecules exceeding 1,000 nucleotides in length, with a 5' cap and a poly (A) tail. To ensure the desired product quality of mRNA therapeutics, critical quality attributes must be maintained within appropriate limits. Therefore, the implementation of robust analytical methods is crucial for making quality decisions.

Attend this webinar for insights into an mRNA characterization workflow that includes sample preparation, ion-pairing reversed-phased liquid chromatography-mass spectrometry (IPRP-LC-MS) methodology, and bioinformatics processing. 

The workflow evaluates 5' cap efficiency, maps oligonucleotide sequence fragments, resolves isomers, and analyzes the intact mass of the poly (A) tail.

Attend this webinar to:

• Explore the LC-MS characterization method for 5' cap, coding sequence mapping, and poly (A) tail analysis
• Gain insights into utilizing ion-pairing reversed-phase LC-MS to characterize mRNA drug substances
• Discover the benefits of utilizing BioTi core-shell column technology to improve sensitivity and resolution for oligonucleotide identification
• Review the bioinformatics processing workflow

Roxana Eggleston

Advanced Workflows Application Manager, Pharma & Biopharma at Phenomenox

Roxana Eggleston-Rangel is the Advanced Workflows Applications Manager, Pharma & Biopharma at Phenomenex. She has worked at Phenomenex for four years, holding other positions including Senior Application Scientist. At Phenomenex, Roxana focuses on expanding the portfolio of biological applications and addressing unique customer needs. Prior to Phenomenex, Roxana worked in developing LC–MS/MS methods, with an emphasis in proteomics at Caltech and University of Southern California. Roxana received her master’s degree in Chemistry & Biochemistry from University of California, Los Angeles, USA.

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SPEAKERS

Roxana Eggleston

Advanced Workflows Application Manager, Pharma & Biopharma at Phenomenox

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