Mastering IP-RPLC-MS for high-throughput & high-resolution oligonucleotide impurity profiling

This webinar, co-hosted by Phenomenex and the Bio-Analytical Separation Science Group at Vrije Universiteit Brussel, will delve into actionable insights into optimizing impurity profiling workflows. The speakers will showcase the potential of ion-pair reversed-phase liquid chromatography coupled with mass spectrometry (IP-RPLC-MS) for high-throughput and high-resolution impurity profiling to enhance the efficacy and safety of oligonucleotide therapeutics. These oligonucleotide therapeutics offer precise gene modulation for tackling rare genetic diseases, with 23 confirmed FDA-approved therapies.

The speaker will explore how base, ribose, and phosphate modifications enhance therapeutic performance by improving binding affinity, thermostability, and nuclease resistance. Further, they will identify and characterize key impurities such as shortmers and phosphodiester byproducts, while exploring methodological strategies to optimize their analysis to ensure more reliable and effective drug development.

Attendees will gain practical insights into optimizing impurity profiling workflows, helping them enhance the quality and safety of their oligonucleotide therapeutics. If you’re involved in CGT development, this session will equip you with actionable tools to streamline your analysis and stay ahead in this rapidly evolving field.

• Learn how base, ribose, and phosphate modifications enhance therapeutic performance through improved binding affinity, thermostability, and nuclease resistance
• Discover strategies to optimize high-resolution impurity profiling for oligonucleotide therapeutics
• Explore the effects of preheater optimization and fluoroalcohol selection on chromatographic performance and mass spectrometry detection
• Evaluate different column materials to improve impurity detection and separation efficiency in your analysis

Sebastiaan Eeltink

Full Professor and Lead Investigator of the Eeltink Laboratory at University of Brussels

Dr. Sebastiaan Eeltink is a Full Professor and lead investigator of the Eeltink Laboratory at the University of Brussel and an Editor of Analytical Science Advances at Wiley. His team focuses on developing and designing novel analytics for the high-resolution profiling of biomolecules and biomolecular interactions using ultrahigh-pressure liquid chromatography (UHPLC) and multi-dimensional liquid chromatographic workflows with mass spectrometry (MS) detection. In addition, his team also manages the University’s proteomics platform which offers high-performance LC-MS proteomics profiling expertise and advise to academic and industrial collaborators.

Quang-Dong Bui

PhD Student at University of Brussels

Quang-Dong Bui is currently a PhD student at University of Brussels under the supervision of Professor Sebastiaan Eeltink, where he develops analytical methods for key oligonucleotide projects and is an active scientist in the oligonucleotide and mRNA research community.

Watch now

SPEAKERS

Sebastiaan Eeltink

Full Professor and Lead Investigator of the Eeltink Laboratory at University of Brussels

Quang-Dong Bui

PhD Student at University of Brussels

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