Better mRNA-LNPs: encapsulation efficiency, mRNA integrity and purity, lipid N-oxides, and beyond

Lipid nanoparticles (LNPs) have emerged as a popular delivery vehicle for gene therapy and vaccines due to their ability to scale up and versatility to encapsulate various sizes of genetic material, amongst other factors. However, LNPs consisting of helper lipids, PEGylated lipids, and ionizable lipids can be susceptible to N-oxide-based impurities that can cause the loss of function of the genetic payload.

To ensure the safety and efficacy of LNP-based drugs, comprehensive characterization of these sophisticated molecules–the lipids and the genetic cargo–is paramount.

Distinguishing harmful N-oxides from other oxidation products is an analytical challenge for most existing technology, especially for ionizable lipids, as they come with various structures consisting of several potential oxidation sites. Furthermore, impurities affecting quality can be present at very low abundance.

In addition, the assessment of LNP encapsulation efficiency, integrity, purity, and stability of in vitro transcribed RNA, such as messenger RNA (mRNA), poses challenges due to its fragile nature, heterogeneity, and length.

In this webinar, Jane Luo, PhD, Senior Scientist, SCIEX will explore these challenges and describe a range of potential solutions, including real-world examples and case studies.

• Learn why process-related lipid impurities are a concern for therapeutics and vaccines
• Discover how to achieve detailed structural characterization of ionizable lipids and their impurities by leveraging more descriptive fragmentation, using the ionizable lipid ALC-0315 as an example
• Explore how to break through limitations of intact mRNA analysis with intuitive, kit-based solutions that offer high-quality integrity and purity results for a wide range of sizes
• Take control of determining mRNA-LNP encapsulation efficiency with an intuitive capillary electrophoresis workflow and achieve the highest resolution power

Jane Luo

Senior Scientist, SCIEX

Dr. Jane Luo is a senior scientist for Cell and Gene Therapy Applications in the Strategic Technical Marketing team at SCIEX. She earned her PhD in Biochemistry from the City University of New York, received postdoctoral training on Molecular Biology and Cell Biology at Weill Cornell Medical College and Harvard Medical School and conducted cancer research as an assistant adjunct professor at UC Irvine. In 2002, she moved to industry to develop capillary electrophoresis-based products and applications.

Watch now

SPEAKERS

Jane Luo

Senior Scientist, SCIEX

You might also like

Pure and simple: understanding LNP analytics for better mRNA-based drugs

Adam Crowe

29 May

Watch

Precise mRNA purity assessment for accelerated therapeutic development and release testing

Paul Mania, Joe Ferraiolo

19 September

Watch

Unlocking mRNA-LNP potential using a novel lipid library combined with innovative analytical methods

Nejc Pavlin, Guillaume Freund

11 September

Watch

Scalable chromatographic strategies for mRNA and saRNA purification from IVT

Tomas Kostelec

29 February

Watch

Beyond the liver: a novel lipid library opening new horizons for LNP developers

Ian Villamagna, Brooke Benson

12 June

Watch