Optimizing downstream purification of high-quality plasmid DNA for gene therapy and vaccine production

The demand for plasmid DNA (pDNA) has increased in recent years, in part due to its utilization in both cell and gene therapies and mRNA therapeutics. Due to the physical properties of these molecules, plasmid production and purification pose some distinct challenges. A design of experiment (DoE) study was conducted in order to evaluate POROS AEX resins for pDNA capture, with the goals of optimizing process conditions to maximize purity and recovery, determine the dynamic binding capacity (DBC) of POROS AEX resins for pDNA, and confirm optimal operating parameters.

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