Reduction of encapsidated hcDNA during AAV production

Watch the video or view the poster to understand why: Apoptotic low molecular weight DNA fragmentation occurs during AAV production The major source of encapsidated hcDNA is apoptotic DNA fragmentation Inhibition of low molecular weight DNA fragmentation reduces the encapsidation of potential oncogenes to an undetectable level New genetic modified HEK293 cell lines reduces encapsidated hcDNA up to 95%

Michelle Hussong is head of the Molecular Biology and Analytics group in the Technology Development department at Cevec—now part of Cytiva. Prior to joining Cevec in 2020, Michelle led research at the University Hospital of Cologne on the (epi)-genetic regulation of long non-coding RNA, and its role in malignant transformation and therapy resistance in lung cancer.

Helmut Kewes is a scientist in the Technology Development department at Cevec—now part of Cytiva—with more than 15 years of experience in cell line development, upstream process development, and viral vector production of AAV-, lentiviral-, and adenoviral vectors, as well as project management.

Please enter your email to access content Read now