Alternative solutions to separate AAV full and empty capsids using anion exchange

AAV is the primary vector for gene therapy, and in order to enable more mainstream applications with larger patient populations, it will prove crucial to have scalable, cost-effective, and resilient chromatography-based purification methods. Central to a successful process is the attainment of high overall yields of full capsids alongside an effective reduction of empty capsids and efficient removal of impurities. This article introduces high-performance anion exchange chromatography separation techniques in step-elution mode for various AAV serotypes, detailing optimized protocols and conditions for both membrane and resin formats.

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