Demystifying AAV affinity capture: mapping AAV-affinity ligand footprints with cryo-electron microscopy

Efficient and cost-effective purification is essential for achieving key objectives in AAV production including maximizing yield, maintaining product quality, and streamlining downstream processes. This poster highlights a study that leverages cryo-electron microscopy (cryo-EM) to map precise interactions between affinity ligands and AAV capsids. Combining robust resin performances—capable of 20+ clean-in-place cycles—with detailed molecular mapping optimizes capsid-resin compatibility, thereby reducing risks, saving time, and ensuring scalable, cost-effective downstream workflows.

Watch the video or read the poster to: learn how cryo-EM mapping reveals precise AAV capsid residues and enables targeted capsid engineering for increased purification outcomes; discover how affinity resins can offer highly efficient, reusable purification with 4-log clearance of host cell proteins and 2.7-log clearance of DNA; and insights into early integration of capsid and resin paring to simplify downstream processes, reducing costs and improving scalability

Dr Nathaniel Clark is a Downstream Scientist at Avitide, a Repligen company. He has a PhD in Biochemistry and Structural Biology from University of Massachusetts Amherst, Amherst, MA, USA and moved into field of RNA as postdoc at the University of Texas. At Avitide, he uses structural biology, protein engineering, and modeling to design novel affinity resins.

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