AAV9 capsid enrichment by optimization of anion exchange chromatography

Watch the video or view the poster to: compare and contrast elution buffer salt systems for enrichment of AAV9 full capsids; find out how to develop and adjust a 3-step elution gradient to increase enrichment and recovery; and effectively harness anion exchange chromatography (AEX) for high-purity AAV manufacture at large-scale.

Angela Andaluz is a dedicated Process Development Scientist at Thermo Fisher Scientific, where she specializes in scaling up processes from bench top to commercial levels. Since joining the company in 2020, Angela has focused on advancing processes for viral vector production, handling both upstream and downstream development across multiple programs. Her expertise in best practices for viral vector development enables her to create and scale-up processes that adhere to cGMP standards. Angela earned her master’s degree in Microbiology and Cell Science from the University of Florida and her bachelor’s degree in Biochemistry from Brigham Young University-Hawaii.

Joshua Orchard is a Field Applications Staff Scientist, specializing in protein and viral vector purification, downstream, process development, and virus clearance. He has held scientific positions at AstraZeneca and Novavax prior to joining ThermoFisher Scientific in 2023. In his 15+ years of work in the industry, Joshua has acquired knowledge of best industry practices and regulatory guidelines and their changes. During this time, he has published peer-reviewed publications in the field of virus clearance. Joshua holds a bachelor’s degree in Biology from the University of North Carolina.

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