A novel PCR-free hybridization method for titering gene therapy vectors in crude cell lysates and various in-process matrices

Maintaining the accuracy and efficiency of AAV genome titer quantification is crucial in gene therapy manufacturing. This poster explores a novel PCR-free assay that enhances speed, reliability, and flexibility. By utilizing DNA hybridization, immunochemistry, and bio-layer interferometry (BLI), this assay simplifies titer quantification and improves workflow efficiency.



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