Scalable AAV manufacturing – Addressing challenges across the workflow

Kenneth Thompson, PhD is a Manager of Cell Biology R&D in the Life Sciences Solutions Group at Thermo Fisher Scientific in Frederick, MD (USA). Dr. Thompson leads a team of scientists focused on developing new products for cell biology applications including Baculovirus-based insect systems for protein expression and virus production. Dr. Thompson received his PhD in Molecular and Cellular Biology and B.S. in Biological Sciences from the University of Maryland, Baltimore County.

Chao Yan Liu is a R&D Senior Manager, Cell Biology at the Life Sciences Solutions Group based in Frederick (MD - USA). Chao Yan Liu received the M.D. degree from the Hebei Medical University (Hebei, China) and the Master degree in Pathology from the Academy of Medical Sciences in Beijing, China. She got her post-doctoral training at Blood Research Institute, Medical school of Wisconsin and then became research assistant professor at the University of Buffalo, New York. After being very active for more than ten years in academic research in the field of cellular biology and immunology, in 2005 she joined the Research and Development department of the former Life Technologies. She has made great contributions to more than ten of the GIBCO® brand Cell Culture Essential products including AlgiMatrix™ 3D Culture System, OpTmizer™ CTS™ T-Cell Expansion SFM, TrypLE™ Select Enzyme, ES Cell Qualified FBS, GlutaMAX™ media supplement, FreeStyle™ F17 Expression Medium and CD FortiCHO™ Medium. She has extensive experiences in mammalian cell culture, serum and protein free, chemical defined medium formulation, as well as protein expression. She served as leading scientist to develop two novel transient transfection systems, Expi293™ and ExpiCHO™ Expression Systems. In the past two years, she and her team focused on both protein expression and viral production system development.

John Li holds a B.S.c. in Biochemistry and a PhD in Structural Biology from the University of Toronto, Canada. Today, John is a Staff Scientist at Thermo Fisher Scientific where he focuses on downstream purification process development for monocolonal antibodies, AAV, and other biotherapeutic modalities.?Before joining Thermo Fisher, John was a Process Development Scientist at Immunogen where he evaluated new downstream platform designs for antibody and antibody drug conjugates.

Michael Brewer is the Director, Global Principal Consultant, Regulatory for the BioProduction Division (BPD) at Thermo Fisher Scientific. In this role, Michael is responsible for providing global support to BioProduction customers and serving as the regulatory thought leader and expert across all technology areas within BPD. Prior to moving to this role, he led the team responsible for product applications including Microbiology, Analytical Sciences and Quality control. The products are fully integrated, solutions for Glycan profiling, Bacterial and Fungal identification, Mycoplasma and Viral detection and host cell DNA and protein quantitation. Michael has over 30 years experience in the Biopharma industry, including, Scios, Synergen and Amgen in a variety of roles including Discovery Research, Analytical Sciences and Quality Control. Prior to joining Thermo Fisher Scientific, he led a group at Amgen that developed qualified, validated and implemented molecular methods for host cell DNA quantitation, contaminant (Mycoplasma, Virus and Bacteria) detection, contaminant identification, strain typing and genotypic verification of production cell lines.