BioInsights - Accelerate process development with at-line concentration measurement
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Accelerate process development with at-line concentration measurement

Accelerate process development with at-line concentration measurement

Currently, the most commonly used methods for adeno-associated viral (AAV) vector quantitative analysis are qPCR and ELISA assays, along with analytical ultracentrifugation (AUC) and transmission electron microscopy (TEM). However, the time to results when using these methods can range from days to several weeks, and the acceptable tolerance range is high. Rapid, reliable in-process testing offers a significant benefit to AAV downstream process development, and can be achieved with Slope Spectroscopy utilizing Variable Pathlength Technology (VPT).

In this webinar, we will demonstrate how the CTech SoloVPE System, when used at-line in one or more stages of a process, can help to quickly identify process characteristics, provide key insights and allow for process optimization in minutes. VPT has enabled at-line measurement of concentration by eliminating the need for sample dilution or manipulation and delivering rapid and highly accurate real-time results.

  • Introduction to Slope Spectroscopy – a different method for UV-Vis concentration measurement
  • Review the Gene Therapy workflow for potential opportunities to accelerate testing to drive process efficiencies
  • Present gene therapy case studies demonstrating the CTech SoloVPE System performance

Joe Ferraiolo

Associate Director, Bioanalytics, Repligen

Joe leads the bioanalytics applications team and is in charge of the SoloVPE variable pathlength spectroscopy system for at-line applications. He has been with the company for more than 20 years, with over ten years of development and validation experience in analytical applications. He specializes in UV analysis and leads the development and commercialization of high-value products and flexible solutions that address critical steps in the production of biologics.


Joe Ferraiolo
Joe Ferraiolo
Associate Director, Bioanalytics at Repligen

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