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To support the development of mRNA-based therapies we have developed a platform chromatography solution for the purification of mRNA. The POROSTMOligo dT(25) affinity resin helps to address the selectivity and capacity requirements for the large-scale manufacturing of mRNA used in vaccine and gene therapy applications. The resin enables a simplified mRNA purification workflow and maximizes the removal of the DNA template and other IVT components.
Typically, mRNA binds to the Oligo dT(25) affinity resin using high ionic strength conditions and neutral pH and is eluted from the column using low ionic strength solutions like water. Although water works well for most mRNA constructs, a need to identify alternative elution buffers which can be used to optimize mRNA recovery, exists. In this webinar you will learn more on how we identified complementary elution buffers by executing a High Throughput Screening (HTS) study.
We evaluated a series of buffers at various pH levels and ionic strengths and determined the recovery. The results from this study will enable a broader understanding of steps that can be taken to optimize affinity chromatography in the mRNA manufacturing process.
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