Large-scale production of hiPSCs in automated bioreactors to produce wholly-cellular bioinks for tissue engineering

Sponsor

Culturing human induced pluripotent stem cells (hiPSCs) suitable for bioprinting applications requires billions of cells, which can differentiate into the desired cell types for tissue engineering. It is challenging to select optimal, reproducible, and scalable process conditions, and culture media, for generating high-yield, functional hiPSCs.

In this webinar, Mark Skylar-Scott, Ph.D., Assistant Professor of Bioengineering at Stanford, and Debbie L. Ho, Basic Life Science Researcher at Stanford, showcase an optimized culture-to-3D bioprinting pipeline for billion cell-scale tissue engineering.

Watch now to:

Learn more about how bioprinting can increase the availability of organs
Explore alternatives to using 2-D flask cultures to generate required cell quantities
Understand how a DoE approach can create efficiencies in your culture process
Learn strategies to maintain hiPSC pluripotency at various bioreactor scales

Mark Skylar-Scott, PhD

Assistant Professor of Bioengineering, Stanford University

Mark Skylar-Scott received his B.A. and M.Eng. degrees in Engineering at the University of Cambridge in 2007. For his doctoral thesis under the guidance of Prof. M. Fatih Yanik at MIT, he developed multiphoton photopatterning techniques to print full length proteins on 2D surfaces and in 3D scaffolds to probe and direct neural and vascular growth. For his postdoctoral research at Harvard and the Wyss Institute with Prof. Jennifer Lewis, he performed 3D bioprinting of thick and vascularized tissues, and created new high-throughput multimaterial multinozzle 3D printing systems. Mark is now an Assistant Professor of Bioengineering at Stanford, a member of the BASE Initiative at the Children’s Heart Center and a Chan-Zuckerberg Biohub Investigator. He received the NIH Director’s New Innovator Award in 2022 towards his goals to develop new 3D printing hardware, wetware, and software to accelerate cardiovascular tissue engineering towards thick, vascularized, and functionally therapeutic organs.

Debbie Lee Lian Ho, MS

Basic Life Science Researcher, Stanford University

Debbie graduated with her MRes in Stem Cell Biology and Medicine and MPhil in Clinical Sciences at the University of Cambridge. She moved to Stanford to work in the Skylar-Scott lab, focusing on the optimization of the scalable culture of human induced pluripotent stem cells and their bioprinting applications.

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