This webinar aims to provide a technical overview of rapid process development for an adeno-associated virus (AAV) capture step, using AVIPure® AAV affinity resins as a case study. A high throughput DOE framework will be shared on how to effectively identify desirable conditions for AAV chromatography. The focus will be on discussing essential elements such as resin screening methodologies, involving rapid elution scouting and refinement through OPUS® RoboColumns® to maximize yield and purity, while ensuring capsid stability.
Strategies for using pre-packed columns will be reviewed, highlighting their potential to enhance process development efficiency by reducing time consumption and improving scalability. We’ll discuss how to implement these columns effectively in various stages of AAV purification to optimize HCP and DNA removal while maximizing yield.
By attending this webinar, participants will gain valuable insights into streamlining their AAV purification steps, enabling them to develop optimal and efficient processes to scale as needed.