Achieving comprehensive analysis and characterization of extracellular vesicles

Wednesday 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

Sponsor

Achieving comprehensive analysis and characterization of extracellular vesicles

Live30 webinars are thirty-minute presentations designed to update you on the latest innovations, applications, and data in a fast yet interactive format.

Accurately characterizing extracellular vesicles (EVs) can be challenging, even in highly purified cell culture samples. Interference from lipoproteins, cell debris, and protein aggregates can pose challenges. In addition, complex biofluids, limited sample volumes, and rare EV subpopulations can all add further complexity.

Leprechaun provides a solution by isolating EVs on its Luni consumable before measuring particle size and concentration, and analyzing EV phenotype for up to 4 surface or cargo markers simultaneously, from <25 µL of sample. It can be applied to a range of materials without the need for sample purification, while providing sensitivity down to 5x10^5 particles/mL, single particle analysis, and the ability to size EVs as small as 35 nm.

In this webinar, you’ll learn how Leprechaun combines single particle interferometry and immunofluorescence imaging to provide comprehensive analysis of any EV sample. Leprechaun can also check loading efficiency of EV packaged cargo, ID whether a protein is soluble or EV-associated, and provide a complete breakdown of all EV subpopulations.

Learn how to perform single particle analysis of EVs and exosomes in crude and pure samples
Discover how to obtain concentration, size, and phenotype information for any subpopulation of EVs
Discover how to analyze protein and nucleic acid EV cargo

Alex Shephard

Market Manager at Unchained Labs

Alex Shephard is the Market Manager for cell therapy at Unchained Labs. She has many years of experience at the bench and as a field application scientist characterizing exosomes and viruses, using techniques including NTA, DLS, nanoflow cytometry, fluorescence microscopy and interferometry. Alex completed a Ph.D. in Molecular Biology at Bristol University, UK, and held a postdoc position in the Tumour Microenvironment Group at Cardiff University, UK.