Measuring key analytical attributes of viral vectors with macro mass photometry
Sponsor
Adenoviruses (AdV) and lentiviruses (LV) are key tools in the cell and gene therapy space, representing some of the most popular viral vector platforms. Accurate characterization of key analytical attributes of viral vector samples throughout the production process is essential for ensuring quality and efficacy. Measuring these attributes, however, presents significant challenges. Conventional vector characterization techniques have considerable limitations–cell-based analyses are time-consuming, requiring days for a result, while faster methods like nanoparticle tracking analysis (NTA) offer only limited data. A lack of efficient, in-process tools creates substantial bottlenecks in the development of cell and gene therapies.
Macro mass photometry is an innovative new approach that streamlines LV vector analysis, offering rapid, accurate assessments that provide critical information for therapy development.
In this webinar, Dr. Laura Pala explains how the KaritroMP macro mass photometer measures the size and contrast (related to mass) of viral vectors at the single-particle level, enabling characterization of purity, populations, and physical titer of viral vector samples with speed, accuracy and no need for labels or other sample modification. Through case studies and benchmarking data, she will demonstrate how macro mass photometry can be used to streamline production and purification methods for cell and gene therapies.
- The advantages of macro mass photometry for characterizing viral vector purity and stability
- Preliminary data on lipid nanoparticles showing a comparison between empty and loaded particles
- How to quickly assess the purity of AdV preparations, quantifying empty capsids and other impurities
- How to quickly monitor the lentivirus enrichment throughout the manufacturing steps, correlating with physical titer
- How to optimize production and purification methods for viral vectors
SPEAKERS
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