Salt-tolerant endonucleases and the benefits for viral vector manufacturing, and a comparison of two marketed enzymes
Sponsor
Live30 webinars are thirty-minute presentations designed to update you on the latest innovations, applications, and data in a fast yet interactive format.
Recombinantly produced Serratia marcescens endonucleases, such as DENARASE® and Benzonase®, are widely used for DNA removal in viral vector production processes. To achieve higher vector yields, manufacturers are increasingly using higher salt concentrations, up to 500 mM, which adversely impacts the activity of S. marcescens endonucleases. Consequently, producers of viral vectors are seeking alternative, more salt-tolerant enzymes for nucleic acid digestion.
In this webinar, we describe the making of DENARASE® High Salt, an engineered variant of the S. marcescens wild-type enzyme, and its potential to streamline viral vector manufacturing processes. In addition, we present test results from an independent, not-for-profit CGT research and technology organization, comparing the new enzyme with another commercially available salt-tolerant endonuclease.
Attend this webinar to:
- The benefits of higher salt concentrations in viral vector manufacturing
- The art and power of enzyme engineering
- How salt-tolerant endonucleases enable leaner viral vector processing
- A performance comparison of commercially available salt-tolerant endonucleases
SPEAKERS
You might also like
Advancing viral vector manufacturing with innovation: the importance of the cell line
Optimizing bioprocess purification: effective nucleic acid removal in high salt environments
Safeguard your viral vector tech transfer: considerations and case studies
