Optimizing lentiviral purification: enhancing chromatography with monoliths

Wednesday 08:00 PST / 11:00 EST / 16:00 GMT / 17:00 CET

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Optimizing lentiviral purification: enhancing chromatography with monoliths

Lentiviral vectors (LVVs) can effectively deliver up to 10 kb of target genes and can infect non-dividing cells, making them a popular vector choice for cell and gene therapies. However, a major drawback of using LVV is its arduous purification process. Current processes either do not remove enough impurities, or lose the majority of the virus during purification.

Monoliths are an ideal tool for the purification of large biomolecules and are now being applied to lentiviral purification, to offer the biotech community a robust process that works.

This webinar describes a systematic approach to using monoliths for LVV purification, including using monoliths in a multi-well plate format with a DOE approach, for faster and parallel testing. With each test, infectious recovery increased, eventually doubling from the start point. The results from the plate format were repeated and validated on preparative scale columns, achieving an average of more than 70% infectious recovery and halving the usual process development time. The economic footprint was lower due to less material used, and due to the reusability of the monolith.

Join the webinar to learn how:

The combination of DOE and the use of monolith in a multi-well plate format can significantly reduce process development time and double the initial recovery
A process developed on multi-well plates was validated on a preparative scale
Monolith technology can save labor hours and material costs

Luka Bevc

Manager Product/Application Area at Sartorius BIA Separations

Luka Bevc earned his bachelor’s and master’s degrees in Biochemistry from the University of Ljubljana and his PhD in Molecular Mechanisms of Disease from the University of Copenhagen. He works as a Project Manager in the Department for Process Development for Viruses at Sartorius BIA Separations, specializing in downstream process development for enveloped viruses. His current focus is on the development and optimisation of downstream process for lentiviruses.