CELL & GENE THERAPY INSIGHTS

Welcome to the Vector Purification Hub

As the cell and gene therapy industry continues to rapidly evolve, efficient, reproducible, scalable, and cost-effective viral vector manufacture is of critical importance. Adenovirus, retrovirus, lentivirus, and adeno-associated virus (AAV) remain the most commonly used vectors for the delivery of in vivo and ex vivo gene therapies, and a successful viral manufacturing pipeline must be able to deliver a consistent, pure, and high-titer product that exhibits good safety and efficacy.

At the same time, non-viral delivery platforms such as mRNA continue to evolve and grow in terms of their utility for the advanced therapies field, presenting novel challenges and opportunities for bioprocess developers.

For the viral vector field in particular, downstream processing requires the purification of virus particles from process-and product - related impurities including host cell material, plasmid DNA, and empty capsids. In addition, downstream processing of both viral vectors and mRNA can represent a significant portion of the total cost of production, so effective methods of generating high purity vector product are crucial.

The following collection of curated content from Thermo Fisher Scientific explores the key role that viral vectors and mRNA play in cell and gene therapy, the current challenges facing the field, and potential solutions for successful vector manufacture.

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Content

Process optimization of mRNA purification for vaccines and therapeutic applications

Process optimization of mRNA purification for vaccines and therapeutic applications

Chantelle Gaskin
13 June 2024
Watch
5 best practices for lentiviral purification and downstream processing success

5 best practices for lentiviral purification and downstream processing success

4 April 2024
Poster
Exosome manufacturing: INFOGRAPHIC

Exosome manufacturing: INFOGRAPHIC

2 April 2024
Infographic
Advancing gene therapy development with a multi-serotype AAV affinity resin

Advancing gene therapy development with a multi-serotype AAV affinity resin

Nicolas Laroudie, Quentin Bazot
30 January 2024
Watch
Affinity chromatography for malaria vaccine purification

Affinity chromatography for malaria vaccine purification

Eugene Sun
25 January 2024
Webinar Digest
Streamlining vaccine development & production with modality-specific purification tools for mRNA & beyond

Streamlining vaccine development & production with modality-specific purification tools for mRNA & beyond

Eugene Sun
22 November 2023
Innovator Insight
Trends and Innovation in Lentiviral Vector Processing

Trends and Innovation in Lentiviral Vector Processing

31 October 2023
EBook
Advancing the purification of VSV-G pseudotyped lentiviral vectors by using affinity chromatography

Advancing the purification of VSV-G pseudotyped lentiviral vectors by using affinity chromatography

Pim Hermans, Frank Detmers
11 August 2023
Webinar Digest
Driving the expansion of mRNA into the therapeutic sphere

Driving the expansion of mRNA into the therapeutic sphere

Alejandro Becerra, Andreas Kuhn, Metin Kurtoglu
13 July 2023
Poster
Enabling rapid vaccine development through manufacturing innovation & process efficiency

Enabling rapid vaccine development through manufacturing innovation & process efficiency

C Kontoravdi, M Muralidhara, S Sikka et al.
21 June 2023
Expert Roundtable
Efficient, scalable purification of VSV-G lentivirus by novel affinity chromatography

Efficient, scalable purification of VSV-G lentivirus by novel affinity chromatography

Frank Detmers
7 June 2023
FastFacts
Advancing the purification of VSV-G pseudotyped lentiviral vectors by using affinity chromatography

Advancing the purification of VSV-G pseudotyped lentiviral vectors by using affinity chromatography

Pim Hermans, Frank Detmers
3 May 2023
Innovator Insight
Simplifying lentiviral downstream processing with a novel affinity resin & robust analytical tools

Simplifying lentiviral downstream processing with a novel affinity resin & robust analytical tools

Suzy Brown
14 March 2023
Webinar Digest
Advancing the purification of VSV-G pseudotyped lentiviral vectors by using affinity chromatography

Advancing the purification of VSV-G pseudotyped lentiviral vectors by using affinity chromatography

Pim Hermans, Frank Detmers
7 March 2023
Watch
Simplifying lentiviral downstream processing with a novel affinity resin & robust analytical tools

Simplifying lentiviral downstream processing with a novel affinity resin & robust analytical tools

Chantelle Gaskin, Suzy Brown
27 February 2023
Innovator Insight
Driving the expansion of mRNA into the therapeutic sphere

Driving the expansion of mRNA into the therapeutic sphere

A Becerra, A Kuhn, M Kurtoglu
23 February 2023
Watch
Optimizing vector production & purification to enhance scalable AAV manufacturing

Optimizing vector production & purification to enhance scalable AAV manufacturing

Jenny England
24 January 2023
Poster
Manufacturing considerations underpinning viral & non-viral platform selection

Manufacturing considerations underpinning viral & non-viral platform selection

A Hagerman, A Noyes, V Slepushkin et al.
15 December 2022
Poster
Optimizing vector production and purification to enhance scalable AAV manufacturing

Optimizing vector production and purification to enhance scalable AAV manufacturing

J Zmuda, C Gaskin, J Molinari et al.
7 December 2022
Innovator Insight
Simplifying lentiviral downstream processing with a novel affinity resin and robust analytical tools

Simplifying lentiviral downstream processing with a novel affinity resin and robust analytical tools

Chantelle Gaskin, Suzy Brown
29 November 2022
Watch
Addressing current challenges in lentiviral vector purification and associated analytics

Addressing current challenges in lentiviral vector purification and associated analytics

Bryan Zee, Anindya Dasgupta
20 September 2022
Watch
Selecting the right elution buffer for mRNA purification using the POROS Oligo (dT)25 Affinity Resin

Selecting the right elution buffer for mRNA purification using the POROS Oligo (dT)25 Affinity Resin

Jenny England
18 September 2022
Poster
POROSTM CaptureSelectTM AAVX elution optimization study for optimized recovery of AAV6 capsids

POROSTM CaptureSelectTM AAVX elution optimization study for optimized recovery of AAV6 capsids

Jenny England
27 August 2022
FastFacts
Optimizing vector production and purification to enhance scalable AAV manufacturing

Optimizing vector production and purification to enhance scalable AAV manufacturing

Chantelle Gaskin, Jon Zmuda PhD
25 August 2022
Watch
Simplifying residual DNA analysis in viral vector production: focus on E1A

Simplifying residual DNA analysis in viral vector production: focus on E1A

12 August 2022
Innovator Insight
How can we maximize the efficiency of large-scale LV vector production?

How can we maximize the efficiency of large-scale LV vector production?

4 August 2022
Poster
Manufacturing considerations underpinning viral and non-viral platform selection

Manufacturing considerations underpinning viral and non-viral platform selection

A Hagerman, A Noyes, V Slepushkin et al.
30 July 2022
Innovator Insight
Mycoplasma testing regulatory guidance and strategies for cGMP cell and gene therapy manufacturing

Mycoplasma testing regulatory guidance and strategies for cGMP cell and gene therapy manufacturing

Michael Brewer
19 July 2022
Watch
Optimizing mRNA purification conditions by using a High Throughput Screening approach

Optimizing mRNA purification conditions by using a High Throughput Screening approach

Jenny England
14 June 2022
Watch
POROS CaptureSelect AAVX Wash Optimization Study for Optimized Recovery and Purity of AAV6 Capsids

POROS CaptureSelect AAVX Wash Optimization Study for Optimized Recovery and Purity of AAV6 Capsids

Jenny England
1 June 2022
FastFacts
Manufacturing considerations for viral and non-viral platform selection

Manufacturing considerations for viral and non-viral platform selection

V Slepushkin , A Noyes, A Hagerman et al.
10 May 2022
Watch
Ebook: Viral Vector Purification

Ebook: Viral Vector Purification

27 March 2022
EBook
Current technological trends & advancements in vector purification

Current technological trends & advancements in vector purification

Y Cai, N Clement, C Gaskin et al.
3 March 2022
Innovator Insight
Optimizing downstream purification of high-quality plasmid DNA with POROS™ Chromatography Resins

Optimizing downstream purification of high-quality plasmid DNA with POROS™ Chromatography Resins

Alejandro Becerra, Johannes Buyel
14 February 2022
Poster
Platform optimization for efficient AAV purification: insights from a CDMO

Platform optimization for efficient AAV purification: insights from a CDMO

Vincent Ravault, Nicolas Laroudie
23 January 2022
Innovator Insight
Current technological trends and advancements in vector purification

Current technological trends and advancements in vector purification

C Gaskin, A Saksule, N Clement PhD et al.
15 December 2021
Watch
Platform optimization for efficient AAV purification – insights from a CDMO

Platform optimization for efficient AAV purification – insights from a CDMO

Nicolas Laroudie, Vincent Ravault
26 October 2021
Watch
Expert Roundtable: evolution in AAV process development: 2022 and beyond

Expert Roundtable: evolution in AAV process development: 2022 and beyond

Matthias Hebben
13 October 2021
Expert Roundtable
Optimizing downstream purification of high-quality plasmid DNA for gene therapy and vaccine production

Optimizing downstream purification of high-quality plasmid DNA for gene therapy and vaccine production

Alejandro Becerra, Johannes F Buyel
3 August 2021
Watch
Key lessons for cell & gene therapy and mRNA therapeutic development from COVID-19 mRNA vaccine approvals

Key lessons for cell & gene therapy and mRNA therapeutic development from COVID-19 mRNA vaccine approvals

S Zobbi, V Indurthi PhD, C Kröner et al.
22 April 2021
Watch
Supporting development of mRNA-based therapies by addressing large scale purification challenges

Supporting development of mRNA-based therapies by addressing large scale purification challenges

Kelly Flook
9 February 2021
Watch
Scalable AAV manufacturing: addressing challenges across the workflow

Scalable AAV manufacturing: addressing challenges across the workflow

18 January 2021
Innovator Insight
Development and validation of quantitative real-time PCR for the detection of residual HEK-293 host cell DNA update

Development and validation of quantitative real-time PCR for the detection of residual HEK-293 host cell DNA update

Kara Norman
20 October 2020
Expert Insight
Downstream purification of adeno-associated virus for large-scale manufacturing of gene therapies

Downstream purification of adeno-associated virus for large-scale manufacturing of gene therapies

Mikhail Goldfarb, Orjana Terova
24 September 2020
Innovator Insight
Expert Roundtable: getting a gene therapy product to market: pitfalls and how to prevent them

Expert Roundtable: getting a gene therapy product to market: pitfalls and how to prevent them

Hetal Brahmbhatt, Thomas Guarinoni, Elizabeth Simmons
22 September 2020
Expert Roundtable Video
AAV downstream processing for industrial scale production – moving towards commercial manufacturing

AAV downstream processing for industrial scale production – moving towards commercial manufacturing

Orjana Terova, Mikhail Goldfarb
20 May 2020
Watch
Development and validation of quantitative real-time PCR for the detection of residual HEK-293 host cell DNA

Development and validation of quantitative real-time PCR for the detection of residual HEK-293 host cell DNA

Kara Norman
27 February 2020
Watch
Accelerating advancement in gene therapy by improving downstream purification of viral vectors

Accelerating advancement in gene therapy by improving downstream purification of viral vectors

Orjana Terova, Hetal Brahmbhatt
27 August 2019
Watch
Upstream and downstream solutions for AAV manufacturing

Upstream and downstream solutions for AAV manufacturing

J Rooij, J DeConto, G Schaenzler et al.
27 August 2019
Innovator Insight
Breakthrough Therapy Designation & Regenerative Medicine Advanced Therapy Designation Programs in Cellular & Gene Therapies

Breakthrough Therapy Designation & Regenerative Medicine Advanced Therapy Designation Programs in Cellular & Gene Therapies

Xiaofei Wang, Wilson W Bryan, Lei Xu
30 July 2018
Expert Insight
Overcoming Downstream Purification Challenges for Viral Vector Manufacturing: Enabling Advancement of Gene Therapies in the Clinic

Overcoming Downstream Purification Challenges for Viral Vector Manufacturing: Enabling Advancement of Gene Therapies in the Clinic

O Terova, P Hermans, J Rooij et al.
25 March 2018
Innovator Insight

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