BioInsights - Optimizing downstream purification of high-quality plasmid DNA with POROS™ Chromatography Resins

Optimizing downstream purification of high-quality plasmid DNA with POROS™ Chromatography Resins

Cell & Gene Therapy Insights 2022; 8(1), 87

10.18609/cgti.2022.029

Published: 14 February 2022
ALEJANDRO BECERRA, Johannes Buyel

The demand for plasmid DNA (pDNA) has increased in recent years, but due to their physical properties there are some inherent challenges to the purification of these molecules. A typical downstream process for plasmids normally has multiple steps after fermentation, and anion exchange followed by hydrophobic interaction chromatography are commonly utilized. Thermo Fisher Scientific has developed a variety of resins well-suited for these steps, designed to simplify workflows and increase purity and yield. A series of experiments were conducted in order to evaluate POROSTM AEX resins for pDNA capture, with the goals of optimizing process conditions to maximize purity and recovery, determining the dynamic binding capacity (DBC) of POROS AEX resins for pDNA, and confirming optimal operating parameters. 

Some highlights of these studies, performed in collaboration with the Fraunhofer Institute for Molecular Biology, Germany, are presented here

To find out more, read the full discussion here.